Journal: Cancer Cell International

Article Title: Human cytomegalovirus interleukin-10 enhances matrigel invasion of MDA-MB-231 breast cancer cells

doi: 10.1186/s12935-017-0399-5

Figure Lengend Snippet: MDA-MB-231 cells exhibit increased migration and matrigel invasion in the presence of cmvIL-10. a Transwell migration toward EGF after 5 h in the presence of conditioned medium from mock or HCMV-infected fibroblasts at the indicated MOIs. IL-10R neutralizing antibody (NAb) was included at 30 μg/ml. b Matrigel invasion toward EGF in the presence of 100 ng/ml purified recombinant cmvIL-10 or hIL-10 after 22 h. c Matrigel invasion toward EGF in the presence or absence of 100 ng/ml purified recombinant cmvIL-10 or conditioned medium from mock or infected fibroblasts (MOI = 1). Where indicated, 10 μM Stat3 inhibitor was included. Error bars SEM. *p < 0.05, **p < 0.001. These results are representative of three independent experiments

Article Snippet: For invasion, 96-well matrigel-coated BD Fluoroblock transwell invasion plates (Corning) were used.

Techniques: Migration, Infection, Purification, Recombinant